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It might feel a little intimidating at first, but following a clear process makes all the difference

Positional cloning of the mouse obese gene and its human homologue

Once thawed: Centrifuge briefly (30 seconds at 10,000 rpm) to collect any condensation from the tube walls Prepare fresh dilutions in the desired experimental buffer (PBS, cell culture media, etc.) Maintain on ice during experiment setup Use within 24 hours of dilution