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2.6 Immunophenotyping by flow cytometry PBMCs were isolated using Ficoll-Paque density gradient centrifugation, frozen in freezing medium [10% DMSO, 90% fetal bovine serum (FBS)] and stored at -80C until use

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These autonomic nervous systems control the functions that our body does automatically, such as our heartbeat, metabolism, digestion, urinary and bowel movements, and breathing

Our experience has shown that the most targeted muscles on the affected side are the platysma, orbicularis oculi, mentalis, levator labii superioris, levator labii superioris alaeque nasi and depressor anguli oris