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polynucleotide shuffling & interrupted synthesis non-stochastic polynucleotide reassembly is used to obtain the library of recombinant nucleic acids, using a variety of substrates to acquire or improve various properties for different applications

Acknowledgements This study was supported by the Ministre de la Recherche et de la Technologie, Inserm and Universit Clermont-Auvergne (UMR1071 - Units Mixtes de Recherche), INRAE (Institut national de la recherche agronomique, USC-1382) and by grants from the Conseil Rgional dAuvergne, CPER (Contrats de Plan tat-Rgion) EPICURE 2016, FRM (Fondation pour la Recherche Mdicale), la Ligue Contre le Cancer 03/15/63/71 and by the French government IDEX-ISITE initiative (Grant number: 16-IDEX-0001-CAP 20-25) of the University of Clermont Auvergne

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Despite this versatility, the lack of a defined molecular recruitment logic renders the packaging process inherently stochastic, leading to significant fluctuations in loading efficiency and a heterogeneous product population consisting of fully loaded particles, empty shells, and unintended surface-adsorbed cargo