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Metabolomics analysis For metabolomics analysis, supernatants from 1 10 6 MCF7 AR and Ad cells ( n = 6 biologically independent samples) were collected and centrifuged at 3000 g for 10 min at 4 C to remove cell debris and the lipid layer, followed by untargeted metabolic profiling by Metabo-Profile Inc (Shanghai, China)

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Symptoms of Vitamin B-12 Deficiency Fatigue and Weakness Low Moods Low Concentration Level Loss of Appetite Constipation Poor Memory Foods Rich in Vitamin B-12 Beef and chicken liver Wild-caught fish such as salmon, trout, and tuna Grass-fed beef and lamb Pasture-raised poultry Organic dairy products like milk and yogurt Who Is Most Likely to Have a Vitamin B-12 Deficiency

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