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Effect of cigarette smoke on DNA damage, oxidative stress, and morphological alterations in mouse testis and spermatozoa

Sequencing was performed using the NextSeq500 (Illumina) as 37-bp paired-end sequencing with 20 million reads

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In addition, LA transactivated the AP1-luc responsive collagenase promoter construct that was transiently transfected in SkBr3 and Ishikawa cells and stimulated the transcriptional activity of reporter plasmids containing the c-fos and p21 Cip1/WAF1 promoter sequences (Figures 4c and d)