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Endogenous peroxidase activity was blocked by incubating sections in methanol/1.5% H 2 O 2 (SigmaAldrich) for 30 min and incubated with blocking solution (3% BSA [SigmaAldrich], 5% donkey serum [Millipore], 1.5% horse serum [Vector Laboratories] 20 mM MgCl 2 , 0.3% Triton [SigmaAldrich] in PBS) for 1 h