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SUGGESTED: Study: World's booziest nations

The cells were lysed for 1 h rotating at 4 C with lysis buffer (50 mM NaCl, 50 mM Tris, 1 mM EDTA pH 7.4, 0.1% NP-40 (Igepal), 1 mM DTT, cOmpleteTM EDTA-free protease inhibitor cocktail (Roche), PhosSTOP phosphatase inhibitors (Roche), 10 g/mL DNAseI and RNAse)