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KD were calculated by fitting the concentration-dependence of the steady-state SPR responses using the Biacore T200 Evaluation Software version 3.1

Purified product was subjected to NMR analysis and analysis using chiral analytical HPLC with a CHIRALPAK IA-3 column (150 4.6 mm, 3 m) at room temperature (flow rate 1 ml min 1 , 40% acetonitrile in H 2 O with 0.1% trifluoroacetic acid)

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Determination of estradiol content Estradiol was assayed using the microplate reader (mindray MR-96A), microplate washer (Mindray MW-12A) and E2 AccuBind ELISA Kit

[12] Fight Autoimmune Problems Glutathione's antioxidant and anti-inflammatory properties can help modulate the immune response and reduce the underlying inflammation associated with autoimmune diseases

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