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For immunofluorescence staining of pancreas, the slides were deparaffinized and subjected to antigen retrieval, then permeabilized, blocked, and incubated with various antibodies, including anti-Glucagon (ab92517, Abcam), anti-Insulin (ab181547, Abcam), anti-Chac2 (16304-1-AP, Proteintech), and anti-MPO (AF3667, R&D systems)

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