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It is known that in the process of ischemia, a cascade of metabolic reactions is activated, some of which are involved in the production of reactive oxygen species (ROS) and reactive nitrogen species (RNS), such as superoxide, hydrogen peroxide [H 2 O 2 ], hydroxyl radical, nitric oxide [NO], and peroxynitrite, which cause most of the damage observed in ischemia-reperfusion [53,54,55,56]

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The following comparison provides the key distinctions relevant to experimental design

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