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[PubMed] [Google Scholar] 66.Anderson M.E., Powrie F., Puri R.N., Meister A

Fifty microliters of stop solution was added to each well and the optical density at 450 nm determined using a microtiter plate reader (Liuyi, Beijing, China) within 15 min

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In fact, the profound ROS accumulation characteristic of sickle RBCs has been demonstrated to be mainly caused by NADPH oxidase, which is regulated by Rac GTPase [179]