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Further work showed that GLP-1 receptor agonists have neuroprotective effects in animal models of AD and PD and re-sensitize insulin signaling (Vaccari et al., 2021)An extensive systematic review and meta-analysis including preclinical studies on the effects of GLP-1 agonists, DPP-4 inhibitors, and dual GLP-1/GIP agonists on NBD, found that liraglutide (Turnes et al., 2018), tested on moderate to severe DA-ergic neuron loss, were not effective in reversing the effects of 6-OHDA on DA-ergic neurons and associated behaviors

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Membranes were then probed with horseradish peroxidase-conjugated secondary antibodies antirabbit IgG (Abcam, A16035, 1:5,000) or antimouse IgG (Abcam, A16011, 1:5,000) and developed using enhanced chemiluminescence substrate (Bio-Rad, 170-5060)

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