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Cells were harvested, and cell numbers were determined, lysed by using liquid nitrogen and 37 C for multiple rapid freeze-thaw cycles, and centrifugated at 10,000 g , 4 C for 10 min

At this stage, epigenetic regulatory mechanisms stabilize the transcriptional activity of hypoxia-inducible factors, upregulate the expression of lipid metabolism enzymes, and significantly enhance cellular membrane sensitivity to oxidative stress (176)

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Thyroid hormones regulate pituitary growth hormone secretagogue receptor gene expression

It was found that circRNA is involved in mitogen-activated protein kinase/extracellular regulation of protein kinase, phosphatidylinositol 3 kinase/protein kinase B, and Wnt/-catenin cell signal transduction pathways and participates in the regulation of ferroptosis in tumor cells 62,63