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Briefly, analysis was performed using Acquity I class UPLC System combined with mass spectrometer Q Exactive Plus Orbitrap (Thermo Fisher Scientific) operated in a negative ionization mode

Petitjean 4 and Arun Sanyal 5 , (1)Pharmanest, (2)Bristol Myers Squibb, (3)Betty and Guy Beatty Center for Integrated Research, Inova Health System, Falls Church, VA, (4)Pharmanest Inc, (5)Division of Gastroenterology, Hepatology, and Nutrition, Virginia Commonwealth University, Richmond, VA Background: Manual histological evaluation of liver biopsy is the gold standard for fibrosis staging in Non-Alcoholic Steatohepatitis (NASH), but it is limited by its inter and intra-reader variability