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Nigellamines, which are the main alkaloids, and polyphenols, such as quercitrin and kaempferol, have been identified in Nigella sativa

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It is possible to repeat these four steps to remove the preceding PLS predictors and add additional PLS predictors, but we choose to consider only one potential PLS predictor for the sake of keeping our model as simple and interpretable as possible and because this methodology is prone to overfitting when multiple PLS components are retained

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