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2.10 Overexpression and knockdown of RRM2 gene in TPC-1 and IHH4 cells For upregulating RRM2 expression in cells, we obtained a full-length RRM2 sequence fragment using a PCR method and subsequentlycloned it into vector LV18, verified by DNA sequencing and transfected 293T cells (see Supplementary Materials for details)

Kim SY, Hwangbo H, Kim MY, Ji SY, Lee H, Kim GY, et al

The LAT subunits are members of the amino acid polyamine cation (APC) superfamily 32 , which exhibit a conserved 5 + 5 inverted topology fold, wherein the first five TM helices are related to the second five helices via a pseudo two-fold symmetry axis running parallel to the plane of the membrane 33

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This ensures maximum absorption at the cellular level, allowing the body to fully benefit from its detoxifying, immune-boosting, and skin-supporting properties

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