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Lowering the pH causes a shift toward the tetrameric form

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Quantification of lactoyl-CoA (lactyl-CoA) by liquid chromatography mass spectrometry in mammalian cells and tissues

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The conditions, such as a 75 mM sodium phosphate buffer (pH 3.0) with 1.7 mM 1-octanesulfonic acid, 25 M EDTA, 10% methanol, and a flow rate of 1 mL/min, are optimized for precision and sensitivity, ensuring accurate neurotransmitter profiling at a column temperature of 25C

doi: 10.1128/spectrum.01730-22 60 FiorentinoGCoppolaAIzzoRAnnunziataABernardoMLombardiAet al