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Microspheres were then resuspended with 0.5 g ml 1 mouse anti-human IgG-PE (Southern Biotech), vortexed for 1 min with a microplate vortex at 3,000 rpm, sonicated for 1 min and then incubated with vigorous shaking for 1 h

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Hydrogels are three-dimensional cross-linked polymer networks that can absorb and retain large amounts of water, meaning that they are not poisonous to humans