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4 x 10 4 cells were seeded for the DMSO condition and 8 x 10 4 cells were seeded for the palbociclib condition

25 L standard solution was mixed with 25 L 50 mM 1-ethyl-3(3-(dimethylamino)propyl)carbodiimide, 25 L 10 mM 1-hydroxy-7-azabenzotriazole in 2-(N-morpholino)ethanesulfonic acid buffer and 25 L 10 mg mL 1 DnsHz

Curcumin improves MMC-based chemotherapy by simultaneously sensitising cancer cells to MMC and reducing MMC-associated side-effects

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We show here that the Cys-34 crevice substantially reduces the rate of serum stabilising maleimide hydrolysis in these conjugates, necessitating reagent optimisation