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A 100 g aliquot of protein from each sample were transferred into eppendorf tube followed by the addition of 400 mM DTT (Sigma Aldrich, Cat# D9779) to 5 mM

The secondary products of lipid peroxidation, such as MDA and 4-HNE, may also be good targets to examine for their roles during ferroptosis

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This synthetic compound consists of two naturally occurring amino acidsaspartic acid and phenylalaninejoined by a methyl ester bond [1]

Reed LJ, Glasper A, de Wet CJ, et al

Clinical studies show measurable wrinkle reduction at 48 weeks of twice-daily application, with results continuing to build through 12 weeks and beyond as long as use is consistent