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To identify possible endogenously derived GSDME inhibitors, we devised a liposome leakage assay, which detects the leakage of terbium (Tb 3+ ) from liposomes incubated with recombinant full-length GSDME and active caspase-3, to detect the pore-formation activity of GSDME N-terminal domain (GSDME-NT) (Fig

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