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(2011) 17 independently demonstrated that N-acetylcysteine amide (NACA) remarkably protected retinal pigmented epithelium (RPE) and photoreceptors against oxidative cell damage and death, in vitro (ARPE-19 cell culture treated with tBHP) and in vivo (light damage mouse model)

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The current standard operating procedure by the European Center for the Validation of Alternative Methods (ECVAM) recommends the use of MTT reduction as parameter to make prediction on the skin irritancy of substances using EpiSkin TM test method, while IL-1 endpoint is regarded as a useful adjunct to MTT assay 48

2016, pp

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