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Methods: Male Wistar rats were subjected to a chow (CHOW) diet or a free-choice (fc) diet of either chow, saturated fat and liquid sugar (fcHFHS), chow and saturated fat (fcHF), or chow and liquid sugar (fcHS) for 4 weeks

The refinement was carried out in several cycles of automated refinement in phenix.refine program [43] and manual model building performed in Coot [44]

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248 In addition, IgG can promote the senescence phenotype of macrophages and microglia, release inflammatory factors such as IL-6 and TNF-, form a systemic chronic inflammatory environment, and accelerate multiorgan aging

NAD+'s true power in energy metabolism becomes clear in the electron transport chain, where NADH turns back into NAD+ in complex I

These processes are tightly regulated by the HPG axis, culminating in the production of spermatozoa from spermatogonial stem cells (SSC) (57)