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Gastrointestinal discomfort, including mild nausea or stomach upset, has been noted in anecdotal accounts, though no severe gastrointestinal effects have been documented in controlled studies

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Then cDNA was synthesized using SuperScript TM IV VILO TM Master Mix (Invitrogen, Waltham, MA, United States) and diluted 101 by DNase/RNase-Free Distilled Water (Invitrogen)

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