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Analytical testing confirms greater than 99.8 % purity , assay content verified at 580 mg, molecular ion mass identified at 307 Da and endotoxin levels below 0.001 EU/mg

MRP subfamily transporters and resistance to anticancer agents

Gjurasin M, Miklic P, Zupancic B, Perovic D, Zarkovic K, Brcic L, et al

The unspecific proteins were washed in 10 mM NaP i at pH 8, 150 mM NaCl, 1 mM GSH, 10% glycerol, 50 mM imidazole, and 0.1% reduced Triton X-100 (Sigma) and rat MGST1 protein was eluted in 350 mM imidazole

Ingesting paraxanthine has a lower half-life, and also far less variation across individuals

Without that foundation, your timing strategy is just guesswork