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45 GPx4, an enzyme that specifically reduces lipid hydroperoxides, was recognized many years ago

(visit source) Serhan CN, Chiang N, Van Dyke TE

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The supernatant was used to detect antioxidant biomarkers, such as the glutathione (GSH), total antioxidant capacity (T-AOC), content and malondialdehyde (MDA) levels, according to the manufacturers instructions (Nanjing Jiancheng Bioengineering Institute, Nanjing, China)

However, cancer cells preferentially express the pyruvate kinase M2 subtype (PKM2), which exists as a low-activity dimer