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The full-length PGRMC1 cDNA fragment containing resistant sequences for shRNA was generated by using the primers (Supplementary Methods), and ligated into the Eco RI and Bam HI sites of the C-terminus of the 3xFLAG-tagged expression vector p3xFLAG CMV14 (Sigma)

Stanton adds, and it's treated with a specific type of antibiotic

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Mani S, Cao W, Wu L, Wang R

They include: Thiamin (B1) - Essential for energy production, carbohydrate metabolism, and nerve function Riboflavin (B2) - Crucial for energy production and antioxidant regeneration Niacin (B3) - Important for circulation, nerve function, and digestion Pantothenic Acid (B5) - Aids in the breakdown of fats and carbohydrates Pyridoxine (B6) - Necessary for protein metabolism and red blood cell formation Biotin (B7) - Supports the health of the skin, hair, nails, and nervous system Folate (B9) - Essential for cell growth and development Cobalamin (B12) - Plays a key role in energy production, red blood cell formation, and DNA synthesis Vitamin C (Ascorbic Acid) Vitamin C is a powerful antioxidant that boosts the immune system and helps with inflammation