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Neumann, M

Species differences have a major influence on the renal excretion of ionized drugs

Glantzounis G, Tsimoyiannis E, Kappas A, Galaris D

administered AAV serotype 8-mediated gene therapy, derived by cloning hABCB4 cDNA into a modified AAV expression cassette, as a single dose to ABCB4 knockout mice and found restoration of biliary phospholipid excretion with normalization of plasma alkaline phosphatase and bilirubin

The RNA pellet was washed with 500 L of 75% ethanol and centrifuged at 7,500 g for 5 min at 4 C, air-dried, and resuspended in RNase-free water

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