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Nevertheless, the association between low levels of ROS and the generation of double-strand breaks remains a topic of debate, partly owing to methodological challenges, such as those encountered with the neutral Comet assay and the use of nonspecific biomarkers for double-strand breaks, such as gamma-H2AX, an early cellular response to the induction of DNA double-strand breaks

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| 2010 Deitelhoff, Nicole

81 , 82 Both extravascular and intravascular hemolysis occurs in G6PD-deficient individuals, the latter giving rise to hemoglobinemia and hemoglobinuria

Cells were then plated onto 384-well assay plates at 20,000 cells/5 l/well