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Blood was collected by submandibular puncture into 1 mL Eppendorf tubes, the plasma was separated and stored in 80 C until used

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Beyond this function, we found that movement and rearrangement of the cytoplasmic segment of TM4 D is associated with a 25 rotation of the horizontal coupling helix 2 (CH2 D ) connecting TM5 D and TM4 D (Fig

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