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Immunofluorescence staining for protein expression (Col-2, MMP-13, p16 ink4a , and HMGB-1) was performed following our previously reported methods [38, 40]

Increasingly, reclaimed water is deployed in DPR, indirect potable reuse (IPR), industrial operations, agricultural irrigation, and landscape maintenance, thereby mitigating pressure on finite freshwater reserves

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The standard research reconstitution practice is dilute acetic acid (0.1 percent, approximately 10 mM) which keeps the peptide in a partially unfolded, non-aggregated state