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Option 1 Fixed On/Off: 4 weeks on, 4 weeks off, repeat

As such, the plasmids pTETA, pTETB, pTETC, pTETATA, pTETBTB, and pTETCTC were constructed and introduced into the ET08-N strain, which is the strain ET08 without plasmid, producing ET09-ET14, respectively

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TEM analysis revealed that the mitochondria in the Model group exhibited disrupted outer membranes, with a reduction or disappearance of mitochondrial cristae