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BS 3 crosslinking assay Purified protein samples were buffer exchanged into a crosslinking-compatible buffer (20 mM HEPES, pH 7.5, 150 mM NaCl) using 30 K Amicon Ultra centrifugal filters (Sigma Aldrich Inc., St

10.1186/s13073-021-00891-1 47

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Finally, most studies are preclinical, based on cell and animal models, and lack valid clinical evidence

In addition to that, the RBD-GFP construct could be detected as a positive control for the specific binding of the antibody (Fig.23)

In order to overcome the limitation, ultrasound stimulated piezoelectric nanoparticle activation came in existence, which is able to generate electricity upon exposure of mechanical deformation and serve as a promising tool in targeted stimulation of cells and tissues [219]