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EBs were washed with PBS/0.1% BSA and mounted onto coverslips using ProLong Gold Antifade Mountant (Thermo Fisher Scientific)

In addition to band 3, other key intrinsic proteins include glycophorin A (GPA), which contributes to the negative surface charge of the RBCs membrane and thereby prevents cell aggregation, and Rh-associated glycoproteins (RhAG), which participate in ion transport to maintain proper RBC hydration (11) (Figure 1)

Verhoef et al, 1994)

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Zhao Q, Liu J, Deng H, Ma R, Liao JY, Liang H, Hu J, Li J, Guo Z, Cai J, Xu X, Gao Z, Su S

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