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Lactate dehydrogenase cytotoxicity assay Cellular death results in the loss of cell membrane integrity and an uncontrolled release of intracellular components into the extracellular space, including the release of cytoplasmic lactate dehydrogenase (LDH)

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Mouse MHC molecules were removed using a 1 h immunoaffinity purification method with H-2K-specific monoclonal antibody 20-8-4S, covalently linked to CNBr-activated sepharose (GE Healthcare)

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