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As early as the 1930s, experiments were done in which these tars were painted on the ear linings of rabbits or the shaved backs of mice and found to cause tumors

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BEAS-2B cells were treated with WS-23 (4.5, 1.5, 0.45 and 0.15 mg/mL) for 8 h, then rinsed with PBS 3 times, fixed with 4% paraformaldehyde for 10 min, then rinsed again 2 with PBS

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