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doi: 10.1111/cmi.12308 7 LevyMKolodziejczykAAThaissCAElinavE

On the contrary, arginine depletion blocks T cells in the G0G1 phase, resulting in reduced proliferation and functional impairment (Oberlies et al., 2009

The samples were lysed by freezing and thawing (three times), followed by centrifugation at 13,000 g for 10 min, and the supernatant was collected for subsequent assays